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显微调制荧光仪Microscopy-PAM

简要描述:利用PAM主机连接显微镜,测量1个或多个细胞的光合作用,不需分离培养即可测量自然水样中特定藻细胞的光合作用。

  • 更新日期:2024-01-30
  • 访  问  量: 2488

详细介绍

Microscopy-PAM **台可以测量单细胞光合作用的荧光仪
Schreiber教授因发明PAM系列调制叶绿素荧光仪而获得首届光合作用协会(ISPR)创新奖


以通用控制单元PAM-Control为主机,连接显微镜,结合荧光配件,可以测量单个细胞或数个细胞的光合作用。

研究对象可以为浮游植物或叶绿体

研究自然水样中的浮游植物生理活性时,一般步骤是:首先分离纯化(多为显微镜下用毛细管挑藻),获得纯种细胞后扩大培养,然后研究其生理活性。这个过程至少需要2-3个月。而且有些在自然条件下经常发生水化/赤潮的微藻,分离后总是无法成功养活(特别是海洋甲藻)。

现在,只需取水样回来,在显微镜视野中找出您感兴趣的1个或几个细胞,就可检测它(们)的光合作用活性,省去了分离培养的步骤,大大节省了时间。对许多至今仍无法分离培养成功的藻类,也可研究其生理活性。
         



部分文献

1. Bulychev AA, Kamzolkina NA, 2006. Effect of action potential on photosynthesis and spatially distributed H+ fluxes in cells and chloroplasts of Chara corallina Russian Journal of Plant Physiology 53: 1-9.

2. Ralph PJ, Larkum AWD, Kühl M, 2005. Temporal patterns in effective quantum yield of individual zooxanthellae expelled during bleaching. Journal of Experimental Marine Biology and Ecology 316: 17-28.

3. Pikulenko MM, Bulychev AA, 2005. Light-triggered action potentials and changes in quantum efficiency of photosystem II in Anthoceros cells Russian Journal of Plant Physiology 52: 584-590.

4. Bulychev AA, van den Wijngaard PWJ, de Boer AH, 2005. Spatial coordination of chloroplast and plasma membrane activities in chara cells and its disruption through inactivation of 14-3-3 proteins. Biochemistry (Moscow) 70: 55-61.

5. Bulychev AA, Kamzolkina NA, Rubin AB, 2005. Effect of plasmalemma electrical excitation on photosystem II activity and nonphotochemical quenching in chloroplasts of cell domains in Chara corallina. Doklady Biochemistry and Biophysics 401: 127-130.

6. Bulychev A, Wijngaard P, Boer A, 2005. Spatial coordination of chloroplast and plasma membrane activities in chara cells and its disruption through inactivation of 14-3-3 proteins Biochemistry (Moscow) 70: 55-61.

7. Villareal TA, 2004. Single-cell pulse amplitude modulation fluorescence measurements of the giant diatom Ethmodiscus (Bacillariophyceae). Journal of Phycology 40: 1052-1061.

8. McMinn A, Hegseth EN, 2004. Quantum yield and photosynthetic parameters of marine microalgae from the southern Arctic Ocean, Svalbard. Journal of the Marine Biological Association of the United Kingdom 84: 865-871.

9. Villareal TA, Morton SL, 2002. Use of cell-specific PAR-fluorometry to characterize host shading in the epiphytic dinoflagellate Gambierdiscus toxicus. Marine Ecology 23: 127-140.

10. Goh C-H, Hedrich R, Nam HG, 2002. Evidence for the functional organization of chloroplasts in adaxial guard cells of Vicia faba leaves by single cell analysis. Plant Science 162: 965-972.

11. Goh C-H, Dietrich P, Steinmeyer R, Schreiber U, Nam H-G, Hedrich R, 2002. Parallel recordings of photosynthetic electron transport and K+-channel activity in single guard cells The Plant Journal 32: 623-630.

12. Szyroki A, Ivashikina N, Dietrich P, Roelfsema MRG, Ache P, Reintanz B, Deeken R, Godde M, Felle H, Steinmeyer R, Palme K, Hedrich R, 2001. KAT1 is not essential for stomatal opening. Proc. Natl. Acad. Sci. USA 98: 2917-2921.

13. Ralph PJ, Gademann R, Larkum AWD, 2001. Zooxanthellae expelled from bleached corals at 33℃ are photosynthetically competent. Marine Ecology Progress Series 220: 163-168.

14. Goh C-H, Hedrich R, Schreiber U, 2001. Osmotic stress induces inactivation of photosynthesis in guard cell protoplasts of Vicia leaves Plant Cell and Physiology 42: 1186-1191.

15. Snel JFH, Dassen HHA, 2000. Measurement of light and pH dependence of single-cell photosynthesis by fluorescence microscopy. Journal of Fluorescence 10: 269-273.

16. Goh CH, Schreiber U, Hedrich R, 1999. New approach of monitoring changes in chlorophyll a fluorescence of single guard cells and protoplasts in response to physiological stimuli Plant Cell and Environment 22: 1057-1070.

17. Schreiber U, 1998. Chlorophyll fluorescence: new instruments for special applications. Garab G, ed. Photosynthesis: Mechanisms and Effects. Dordrecht: Kluwer Academic Publishers.

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