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水样荧光仪Water-PAM

简要描述:用于测量自然水样或(培养的)微藻的光合活性,可测量叶绿素含量。三探头可选:系统I用于测量水样;系统II用于测量附着藻类/大型藻类;系统III采用

  • 更新日期:2024-01-30
  • 访  问  量: 5381

详细介绍

schreiber教授因发明pam系列调制叶绿素荧光仪而获得首届光合作用协会(ispr)创新奖

利用调制叶绿素荧光技术,测量野外自然水体或培养的微藻样品的光合作用(叶绿素荧光诱导加淬灭分析、光响应曲线等),也可测量叶绿素含量,是进行野外光合作用研究的良好工具。除了测浮游植物外,可扩展探头测量附着藻类或大型藻类。除了取水样到样品杯中测量外,可扩展探头进行水下原位、连续测量,特别适合于连续监测海洋、湖泊、水库、河流等水体的叶绿素含量以及光合活性。

功能
1)可单机操作,可连接电脑操作
2)可测叶绿素荧光诱导曲线并进行淬灭分析
3)可测光响应曲线和快速光曲线(rlc)
4)可测量水样的量子产量(光合效率)和相对电子传递速率(光合速率)
5)51个内置模式菜单,方便参数设置和标准测量
6)灵敏度高,检测限为0.1 μg l-1 chl
7)三种配置:
系统i用于浮游植物研究;系统ii用于附着藻类和大型藻类研究;系统iii用于连续监测水体光合作用
8)主机为通用控制单元pam-control,可扩展为microscopy-pam和microfiber-pam

通用控制单元pam-control


测量参数
fo、fm、fv/fm、f、fm"、δf/fm"、fo"、qp、qn、npq、retr和par等。

应用领域
水生生物学、水域生态学、海洋学、湖沼学、微藻生理学、微藻抗逆性、水质管理、饮用水源监测、极地藻类研究、环境科学、生态毒理学等。


系统组成

系统i
浮游植物版

系统ii
附着藻类/大型藻类版

系统iii
连续监测版

野外现场自然水体的光合作用检测、叶绿素含量测定;室内培养的微藻样品的生理特性研究等。 野外现场附着藻类(如底泥中的藻类)、大型海藻的光合活性测量;室内大型海藻生理特性研究。 野外现场水体光合活性、叶绿素含量的连续测定,包括垂直梯度分布和横向分布。
可选附件1:搅拌器,可置于系统i的上部对水样进行搅拌,带内置电池

可选附件2:球状微型光量子探头,可放入系统i的样品杯中测量par,也可放入水面下测量水体光场


技术参数
测量光:红色led,650 nm,zui大光强 <1 μmol m-2 s-1
光化光:红色led,660 nm,zui大光强2000 μmol m-2 s-1 par
饱和脉冲:红色led,660 nm,光强4000 μmol m-2 s-1 par
远红光:led,730 nm
信号检测:光电倍增管,检测波长 > 710 nm,选择性锁相放大器(设计),过载保护功能

部分文献

[1] drath m, kloft n, batschauer a, marin k, novak j, forchhammer k. ammonia triggers photodamage of photosystem ii in the cyanobacterium synechocystis sp. strain pcc 6803. plant physiology 2008:in press.
[2] gao k, li p, watanabe t, helbling ew. combined effects of ultraviolet radiation and temperature on morphology, photosynthesis, and dna of arthrospira (spirulina) platensis (cyanophyta). journal of phycology 2008:in press.
[3] hill r, ralph pj. impact of bleaching stress on the function of the oxygen evolving complex of zooxanthellae from scleractinian corals. journal of phycology 2008;44 (2):299-310.
[4] mojaat m, foucault a, pruvost j, legrand j. optimal se-lection of organic solvents for biocompatible extraction of β-carotene from dunaliella salina journal of biotechnology 2008;133 (4):433-41.
[5] bailey s, melis a, mackey krm, cardol p, finazzi g, dijken gv, berge gm, arrigo k, shrager j, grossman a. alternative photosynthetic electron flow to oxygen in marine synechococcus biochimica et biophysica acta 2007;1777 (3):269-76.
[6] ekelund nga, aronsson ka. changes in chlorophyll a fluorescence in euglena gracilis and chlamydomonas reinhardtii after exposure to wood-ash environmental and experimental botany 2007;59 (1):92-8.
[7] geoffroy l, gilbin r, simon o, floriani m, adam c, pradines c, cournac l, garnier-laplace j. effect of selenate on growth and photosynthesis of chlamydomonas reinhardtii aquatic toxicology 2007;83 (2):149-58.
[8] hill r, ralph pj. post-bleaching viability of expelled zooxanthellae from the scleractinian coral pocillopora damicornis. marine ecology progress series 2007;352:137-44.
[9] kromkamp jc, perkins r, dijkman n, consalvey m, andres m, reid rp. resistance to burial of cyanobacteria in stromatolites. aquatic microbial ecology 2007;48 (2):123-30.
[10] kudela rm, ryan jp, blakely md, lane jq, peterson td. linking the physiology and ecology of cochlodinium to better understand harmful algal bloom events: a comparative approach harmful algae 2007;7 (3):278-92.
[11] leu e, falk-petersen s, hessen do. ultraviolet radiation negatively affects growth but not food quality of arctic diatoms. limnology and oceanography 2007;52 (2):787-97.
[12] mcminn a, ryan kg, ralph pj, pankowski a. spring sea ice photosynthesis, primary productivity and biomass distribution in eastern antarctica, 2002-2004 marine biology 2007;151 (3):985-95.
[13] perkins rg, kromkam jc, reid rp. importance of light and oxygen for photochemical reactivation in photosynthetic stromatolite communities after natural sand burial. marine ecology progress series 2007;349:23-32.
[14] ralph pj, ryan kg, martin a, fenton g. melting out of sea ice causes greater photosynthetic stress in algae than freezing in. journal of phycology 2007;43 (5):948-56.
[15] richter p, helbling w, streb c, häder d-p. par and uv effects on vertical migration and photosynthesis in euglena gracilis. photochemistry and photobiology 2007;83 (4):818-23.
[16] roleda my, zacher k, wulff a, hanelt d, wiencke c. photosynthetic performance, dna damage and repair in gametes of the endemic antarctic brown alga ascoseira mirabilis exposed to ultraviolet radiation. austral ecology 2007;32 (8):917-26.
[17] schmitt-jansen m, altenburger r. the use of pulse-amplitude modulated (pam) fluorescence-based methods to evaluate effects of herbicides in microalgal systems of different complexity toxicological and environmental chemistry 2007;89 (4):665-81.
[18] veldhuis mjw, timmermans kr. phytoplankton dynamics during an in situ iron enrichment experiment (eisenex) in the southern ocean: a comparative study of field and bottle incubation measurements. aquatic microbial ecology 2007;47 (2):191-208.
[19] wulff a, zacher k, hanelt d, al-handal a, wiencke c. uv radiation - a threat to antarctic benthic marine diatoms? antarctic science 2007;20:13-20.
[20] zacher k, roleda my, hanelt d, wiencke c. uv effects on photosynthesis and dna in propagules of three antarctic seaweeds (adenocystis utricularis, monostroma hariotii and porphyra endiviifolium) planta 2007;225 (6):1505-16.
[21] 梁英, 尹翠玲, 江新琴, 于云芝. 硅浓度对纤细角毛藻和三角褐指藻生长及叶绿素荧光特性的影响. 海洋水产研究 2007;28 (5):89-94.
[22] 尹翠玲, 梁英, 张秋丰. 磷浓度对球等鞭金藻3011和8701叶绿素荧光特性及生长的影响. 海洋湖沼通报 2007:88-95.
[23] cosgrove j, borowitzka m. applying pulse amplitude modulation (pam) fluorometry to microalgae suspensions: stirring potentially impacts fluorescence. photosynthesis research 2006;88 (3):343-50.
[24] leu e, færøvig pj, hessen do. uv effects on stoichiometry and pufas of selenastrum capricornutum and their consequences for the grazer daphnia magna. freshwater biology 2006;51 (12):2296-308.
[25] peers g, price nm. copper-containing plastocyanin used for electron transport by an oceanic diatom nature 2006;441:341-4.
[26] roleda my, hanelt d, wiencke c. exposure to ultraviolet radiation delays photosynthetic recovery in arctic kelp zoospores. photosynthesis research 2006;88 (3):311-22.
[27] roleda my, wiencke c, lüder2 uh. impact of ultraviolet radiation on cell structure, uv-absorbing compounds, photosynthesis, dna damage, and germination in zoospores of arctic saccorhiza dermatodea journal of experimental botany 2006;57 (14):3847-56.
[28] 梁英, 冯力霞, 尹翠玲, 曹春晖. 高温胁迫对三角褐指藻和纤细角毛藻叶绿素荧光动力学的影响. 中国海洋大学学报 2006;36 (3):427-33.
[29] 梁英, 冯力霞, 田传远, 尹翠玲. 盐胁迫对塔胞藻生长及叶绿素荧光动力学的影响. 中国海洋大学学报 2006;36 (5):726-32.
[30] 冉春秋, 张卫, 虞星炬, 金美芳, 邓麦村. 解偶联剂cccp对莱茵衣藻光照产氢过程的调控. 高等学校化学学报 2006;27 (1):62-6.
[31] barranguet c, veuger b, van beusekom sam, marvan p, sinke jj, admiraal w. divergent composition of algal-bacterial biofilms developing under various external factors. european journal of phycology 2005;40 (1):1-8.
[32] mcminn a, pankowski a, delfatti t. effect of hyperoxia on the growth and photosynthesis of polar sea ice microalgae. journal of phycology 2005;41 (4):732-41.
[33] mcminn a, hirawake t, hamaoka t, hattori h, fukuchi m. contribution of benthic microalgae to ice covered coastal ecosystems in northern hokkaido, japan. journal of the marine biological association of the uk 2005;85:283-9.
[34] mcminn a, sellah s, llah wawa, mohammad m, merican fms, omar wmw, samad f, cheah w, idris i, sim yk, wong ws, tan sh, yasin z. quantum yield of the marine benthic microflora of near-shore coastal penang, malaysia. marine and freshwater research 2005;56 (7):1047-53.
[35] ralph pj, mcminn a, ryan kg, ashworth c. short-term effect of temperature on the photokinetics of microalgae from the surface layers of antarctic pack ice. journal of phycology 2005;41 (4):763-9.
[36] serôdio j, vieira s, cruz s, barroso f. short-term variability in the photosynthetic activity of microphytobenthos as detected by measuring rapid light curves using variable fluorescence. marine biology 2005;146 (5):903-14.
[37] mcminn a, hegseth en. quantum yield and photosynthetic parameters of marine microalgae from the southern arctic ocean, svalbard. journal of the marine biological association of the united kingdom 2004;84:865-71.
[38] ryan kg, ralph p, mcminn a. acclimation of antarctic bottom-ice algal communities to lowered salinities during melting. polar biology 2004;27:679-86.
[39] jones rj, heyward aj. the effects of produced formation water (pfw) on coral and isolated symbiotic dinoflagellates of coral. marine and freshwater research 2003;54 (2):153-62.
[40] sauer jr, schreiber u, schmid r, völker u, forchhammer k. nitrogen starvation-induced chlorosis in synechococcus pcc 7942. low-level photosynthesis as a mechanism of long-term survival. plant physiology 2001;126:233-43.

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